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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of the gene has actually been noticed in many tumors, which results in chromosome instability, and therefore could contribute to tumorigenesis. This protein was discovered to affiliate with, and be linked to, the phosphorylation of NPAT protein (nuclear protein mapped on the ATM locus), which participates in cell-cycle controlled histone gene expression and plays a crucial role in marketing cell-cycle progression during the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinctive isoforms, are already explained. Two more splice variants have been claimed but specific nucleotide sequence data just isn't still available.

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Blocking peptides are peptides that bind specifically towards the goal antibody and block antibody binding. These peptide commonly consists of the epitope recognized from the antibody.

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This guide outlines further doable optimization of cellular and immunoassay parameters to be sure the very best effects are...

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This guide outlines even more feasible optimization of mobile and immunoassay parameters to make certain the absolute best outcomes are...

Antibodies certain to the blocking peptide no more bind towards the epitope to the focus on protein. By evaluating the staining from the blocked antibody compared to the antibody by itself, you can see which staining is unique.

Target Data Cyclin E belongs into the extremely conserved cyclin household, whose members are characterised by a spectacular periodicity in protein abundance with the cell cycle. Cyclins operate as regulators of CDK kinases. Unique cyclins exhibit distinct expression and degradation styles which add on the temporal coordination of each mitotic occasion. Cyclin E varieties a posh with and functions as a regulatory subunit of CDK2, whose exercise is needed for cell cycle G1/S changeover. This protein accumulates on the G1-S phase boundary which is degraded as cells progress through S section.

The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by the two antibodies...

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